ISSN : 1598-6616(Print)
ISSN : 2287-6731(Online)
ISSN : 2287-6731(Online)
Journal of Korean Society of Odor Research and Engineering Vol.9 No.4 pp.203-207
미생물 생균제를 이용한 돈분 악취 저감 연구
Abstract
Occurrence of livestock malodor cause to increase in health impact and thus to increase in public interest. Therefore scientific methods to decrease odor is required. Decrease of odor from cow and pig excreata, however, has not fully been successful. The purpose of this research was to select and assess the microorganism can reduce NH₃ produced from cow excreata. Microorganisms which having potential to decrease odor were isolated from cow excreata The strain NIAST-1 which can decrease odor material, was isolated from among the samples of livestock farm located in Yang-phyang. The isolated strain was identified as Pseudomonas stutzeri with respect to its physiological characteristics and 16s RNA sequencing method. Cell-free supernatant was found to have good and stable odor removing activity. Optimal condition and composition of culture medium were determined as follows: glucose (anhydride) 1.2% (w/v), yeast extract 0.6% (v/v), and temperature (28 ℃), agitaion rate (180 rpm) and aeration rate(0.1 L/min). In field test, microbial agents composed of ASA(Pseudomonas stutzerie) showed marked removal activity of ammonia gas (25%) and hydrogen sulfide (14.5%) gas produced from five ton scale pig storage container. Also this study focussed to assay the characteristics and utilization of environment friendly agricultural materials at laboratory and greenhouse.
