ISSN : 1738-4125(Print)
ISSN : 2287-7509(Online)
ISSN : 2287-7509(Online)
HPLC 분석기법에 기초한 아세톤과 아크롤레인의 피크분리 기초 연구
A Study on the Peak Separation of Acetone and Acrolein based on HPLC method
Abstract
To resolve coelution phenomenon of acetone and acrolein in the HPLC‐based analysis of carbonyls, weattempted to find the optimal conditions for their separation. For the purpose of this study, the collection ofcarbonyl Compounds is made by DNPH‐coated cartridges. Quantification of carbonyls is then initiated by theformation of hydrazone derivatives that are then separated by HPLC and detected by UV‐VIS spectroscopicdetector (at 360 nm). To the course of this experiment, we examined the influences of the three majorexperimental variables such as 5 temperature (25, 30, 40, 50 and 60℃), 2 flow rate (1.0 and 1.2 mL min‐1),and variable relative composition of mobile phase (among acetonitrile, water, and tetrahydrofuran). Accordingto our experiments, the optimal condition for separation was found at flow rate of 1.2 mL min‐1 andtemperature of 32℃ with the mobile phase composition of acetonitrile:water:tetrahydrofuran = 34 : 52.8 :13.2.
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