ISSN : 1738-4125(Print)
ISSN : 2287-7509(Online)
ISSN : 2287-7509(Online)
석면섬유 자동계수를 위한 고효율 현미경법 개발
Development of High-throughput Microscopy (HTM) for Automated Counting of Asbestos Fibers
Abstract
Since it has been reported that asbestos fibers cause serious health problems such as lung cancer,malignant mesothelioma and other related diseases, it turns into social issue leading to a number of studiesfor characterizing asbestos found in the indoor environment. Among the established methods for detectingasbestos fibers, phase contrast microscopy (PCM) method is widely used as it dose not require complicatedprocess nor high-priced equipments. However, PCM method is hard to define a sort of asbestos and todetect tiny asbestos fibers. We developed an image-based high-throughput microscopy (HTM) for automatedcounting of asbestos fibers which were distinguishable from the spherical particles. HTM method enabled usto analyze asbestos fibers both automatically and quantitatively. Test samples of chrysotile, amosite andcrocidolite, which are frequently detected in Korea, were used in this study and comparisons were madebetween concentrations of asbestos fibers measured by manual counting method and HTM method.Application of HTM system can be extended to various areas such as malaria diagnosis, rare cell detectionand bacterial colony counting.
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